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Chapter 2: Data Curation

Chapter 2: Data Curation

Learning goals

This chapter will help you to understand why input data curation is critical for cryoEM/ET, what happens when ‘bad’ data are not removed, and the appropriate parameters to ensure proper data ‘cleaning.’

Expected outcomes

At the end of this chapter, you will:

  • Understand why bad micrographs and bad particles must be removed ahead of 3D analysis
  • How tomographic reconstructions are affected by improper fiducial alignment

Recommended online materials

Contrast transfer function (CTF)

Guiding questions: What is defocus & why do we need to correct for it?

Single-particle analysis

Sample preparation considerations

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