Philosophy: Our goal is to provide a starting point for learners to be introduced to the concepts of cryo-EM processing. We will connect our text with the primary literature and online content from other resources, such as and Finally, we suggest and provide hands-on analyses to help learners explore how changing parameters affect the results.

Chapter 1: Preprocessing


Chapter 2: Data curation

Chapter 3: 3D analysis

Chapter 4: Validation

What you can expect to learn

  • An introduction to practical aspects of cryo-EM processing with RELION
  • Understand how changes in input parameters affect analysis outcomes
  • Understand how data quality affects downstream processing steps
  • How to assess cryo-EM output data