ACA Workshop 2022
Workshop 4: Hands-on Single-Particle Cryo-EM Data Analysis with cryoEDU
Instructors:
- Michael Cianfrocco, Ph.D. (University of Michigan)
- Mark Herzik, Ph.D. (University of California – San Diego)
- Melody Campbell, Ph.D. (University of Washington)
Assistants:
- Yilai Li, Ph.D. (University of Michigan)
- Marc Morizono (University of California – San Diego)
Agenda
9 am – Introduction – Michael Cianfrocco
9:15 am Practical introduction to single particle analysis – Melody Campbell
10:00 am Intro to simulator & motion correction + CTF – Marc Morizono
10:45 am – Coffee break
11:00 am – Sponsor presentation – Joyce Frank (Mitegen)
11:15 am – Auto-picking – Michael Cianfrocco
12:00 pm – Lunch (provided)
Assignment: Independent use of RELION: MotionCorrection > CTF Estimation > AutoPicking
1:30 pm – Recap on assignment
2:00 pm – Particle extraction & 2D classification – Mark Herzik
3:00 pm – Break
3:15 pm – Assignment: Find best set of parameters that yield most particles below 15Å
4:15 pm – Wrap-up – Mark Herzik & Michael Cianfrocco
4:30 pm – Joint happy hour with cryo-ET workshop and national centers workshop
Glossary
We have created a draft glossary of terms to help navigate the jargon of cryo-EM. Download link.
How to use RELION simulator
Introduction to the RELION simulator
Quiz questions
ACA CryoEDU Workshop – Quiz #1 Motion Correction, Contrast Transfer Function, and Particle Picking
ACA CryoEDU Workshop – Quiz #2 Particle Extraction and 2D Classification
https://docs.google.com/forms/d/16A-IK0ZW_NwA_KYRJ_Q-Va5hHkI6HaM29DPeYUrEPok/edit
To learn more about Google Cloud Platform:
Link: https://cloud.google.com/edu/researchers
Contact – Astitva Chopra