ACA Workshop 2022

Workshop 4: Hands-on Single-Particle Cryo-EM Data Analysis with cryoEDU

Instructors:

  • Michael Cianfrocco, Ph.D. (University of Michigan)
  • Mark Herzik, Ph.D. (University of California - San Diego)
  • Melody Campbell, Ph.D. (University of Washington)

Assistants:

  • Yilai Li, Ph.D. (University of Michigan)
  • Marc Morizono (University of California - San Diego)

Agenda

9 am - Introduction - Michael Cianfrocco

9:15 am Practical introduction to single particle analysis - Melody Campbell

10:00 am Intro to simulator & motion correction + CTF - Marc Morizono

10:45 am - Coffee break

11:00 am - Sponsor presentation - Joyce Frank (Mitegen)

11:15 am - Auto-picking - Michael Cianfrocco

12:00 pm - Lunch (provided)

Assignment: Independent use of RELION: MotionCorrection > CTF Estimation > AutoPicking

1:30 pm - Recap on assignment

2:00 pm - Particle extraction & 2D classification - Mark Herzik

3:00 pm - Break

3:15 pm - Assignment: Find best set of parameters that yield most particles below 15Å

4:15 pm - Wrap-up - Mark Herzik & Michael Cianfrocco

4:30 pm - Joint happy hour with cryo-ET workshop and national centers workshop

 

Glossary

We have created a draft glossary of terms to help navigate the jargon of cryo-EM. Download link.

 

How to use RELION simulator

Introduction to the RELION simulator

Quiz questions

ACA CryoEDU Workshop - Quiz #1 Motion Correction, Contrast Transfer Function, and Particle Picking

ACA CryoEDU Workshop - Quiz #2 Particle Extraction and 2D Classification

https://docs.google.com/forms/d/16A-IK0ZW_NwA_KYRJ_Q-Va5hHkI6HaM29DPeYUrEPok/edit

 

 

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GCP Research One Sheeter

Contact - Astitva Chopra