ACA Workshop 2022
Workshop 4: Hands-on Single-Particle Cryo-EM Data Analysis with cryoEDU
Instructors:
- Michael Cianfrocco, Ph.D. (University of Michigan)
- Mark Herzik, Ph.D. (University of California - San Diego)
- Melody Campbell, Ph.D. (University of Washington)
Assistants:
- Yilai Li, Ph.D. (University of Michigan)
- Marc Morizono (University of California - San Diego)
Agenda
9 am - Introduction - Michael Cianfrocco
9:15 am Practical introduction to single particle analysis - Melody Campbell
10:00 am Intro to simulator & motion correction + CTF - Marc Morizono
10:45 am - Coffee break
11:00 am - Sponsor presentation - Joyce Frank (Mitegen)
11:15 am - Auto-picking - Michael Cianfrocco
12:00 pm - Lunch (provided)
Assignment: Independent use of RELION: MotionCorrection > CTF Estimation > AutoPicking
1:30 pm - Recap on assignment
2:00 pm - Particle extraction & 2D classification - Mark Herzik
3:00 pm - Break
3:15 pm - Assignment: Find best set of parameters that yield most particles below 15Å
4:15 pm - Wrap-up - Mark Herzik & Michael Cianfrocco
4:30 pm - Joint happy hour with cryo-ET workshop and national centers workshop
Glossary
We have created a draft glossary of terms to help navigate the jargon of cryo-EM. Download link.
How to use RELION simulator
Introduction to the RELION simulator
Quiz questions
ACA CryoEDU Workshop - Quiz #1 Motion Correction, Contrast Transfer Function, and Particle Picking
ACA CryoEDU Workshop - Quiz #2 Particle Extraction and 2D Classification
https://docs.google.com/forms/d/16A-IK0ZW_NwA_KYRJ_Q-Va5hHkI6HaM29DPeYUrEPok/edit
To learn more about Google Cloud Platform:
Link: https://cloud.google.com/edu/researchers
Contact - Astitva Chopra